| ComplexioLyte solubilisation buffers |
 |
ApplicationsComplexioLytes are optimized ready-to-use buffers for solubilisation of membrane proteins and protein complexes successfully applied in affinity purifications, native gel electrophoresis and denaturing FFE. Target protein examples comprise >100 channel, >20 receptor (including GPCRs) and several transporter target proteins, as well as many membrane-associated proteins. Source materials include membrane preparations from culture cells, immune/blood cells, tissues from the central and peripheral nervous system, muscle (bladder, heart, skeletal), kidney, liver, Xenopus oocytes, mitochondria and "lipid rafts".Please note that ComplexioLyte buffers - as all other materials offered by Logopharm - are strictly for in vitro R&D use only, not for animal studies, drug, household, or other uses. Testing assays
FormulationComplexioLytes contain salts, pH buffer, detergents and certain additives (all high quality grade chemicals) depending on the projected applications. Solutions have been filtered (0.2 μm) and frozen in liquid nitrogen (-20°C for longer term storage) in aliquots of 10 ml. Each buffer may be complemented by a corresponding dilution and/or washing solution (add the extension "d" to the order number). Protease or phosphatase inhibitors and chelators have to be supplemented as required.Ordering and shipmentBuffers for specific applications can be ordered by fax or This e-mail address is being protected from spambots. You need JavaScript enabled to view it . A list of available detergent buffers and their potential applications is shown below. Order numbers are composed as follows:CL-ComplexioLyte number - number of aliquots extension Example: CL-48-01d = 1 x 10 ml of ComplexioLyte 48 with dilution solution. Regular quantities (10-100 ml) are shipped within 5-10 working days on dry ice through FEDEX.
|